cb 2 shrna Search Results


cb 2 shrna  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology cb 2 shrna
(A) Enhanced immunoprecipitation of the ERK1/2 (Lane 4) compared to vehicle-treated controls (Lane 3). Negative controls (Lanes 5 and 6) received the same concentration of β-Arrestin 2 antibody except that the coupling resin was replaced with control agarose resin that is not amine reactive. All columns were incubated with prefrontal cortex lysate (300 μg) from vehicle (Lanes 3 and 5 ) or CP55940 (Lanes 4 and 6) treated rats. Prefrontal cortex lysate (30 μg of protein) was used as an input control (Lane 1 and 2). (B) Increased pERK protein levels in CP55940 treated rats compared to vehicle treated rats. **p<0.01, significant effect of CP55940 treatment compared to vehicle-treated controls. (C) Increased membrane associated β-Arrestin 2 protein levels in PFCx of CP55940 treated rats. **p<0.01 significant effect of CP55940 treatment compared to vehicle-treated controls. (D) CP55940 treatment does not affect total ERK1/2 expression in the PFCx. (E) Increased β-Arrestin 2 mRNA levels in PFCx of CP55940 treated rats. *p<0.01 significant effect of CP55940 treatment compared to vehicle treated controls. (F) β-Arr2 <t>shRNA</t> lentivurs transfection prevents GP1a-induced increases in 5-HT2A receptor mRNA. **p<0.01, significant effect of GP1a treatment on 5-HT receptor mRNA levels in control shRNA tranfected cells compared 2A to vehicle-treated controls. ##p<0.01, significant effect of β-Arr2 shRNA transfection on the GP1a-induced upregulation of 5-HT2A receptors. Representative Western blots are shown in this figure and IOD was calculated as described in Experimental Procedures. The data represent mean ± SEM (n=6–8).
Cb 2 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cb2 shrna (h) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CB2 gene silencing results, individual duplex components or plasmids are also available upon request. Suitable
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cb2 shrna (r) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CB2 gene silencing results, individual duplex components or plasmids are also available upon request.
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cb2 shrna (m) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of CB2 gene silencing results, individual duplex components or plasmids are also available upon request.
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Image Search Results


(A) Enhanced immunoprecipitation of the ERK1/2 (Lane 4) compared to vehicle-treated controls (Lane 3). Negative controls (Lanes 5 and 6) received the same concentration of β-Arrestin 2 antibody except that the coupling resin was replaced with control agarose resin that is not amine reactive. All columns were incubated with prefrontal cortex lysate (300 μg) from vehicle (Lanes 3 and 5 ) or CP55940 (Lanes 4 and 6) treated rats. Prefrontal cortex lysate (30 μg of protein) was used as an input control (Lane 1 and 2). (B) Increased pERK protein levels in CP55940 treated rats compared to vehicle treated rats. **p<0.01, significant effect of CP55940 treatment compared to vehicle-treated controls. (C) Increased membrane associated β-Arrestin 2 protein levels in PFCx of CP55940 treated rats. **p<0.01 significant effect of CP55940 treatment compared to vehicle-treated controls. (D) CP55940 treatment does not affect total ERK1/2 expression in the PFCx. (E) Increased β-Arrestin 2 mRNA levels in PFCx of CP55940 treated rats. *p<0.01 significant effect of CP55940 treatment compared to vehicle treated controls. (F) β-Arr2 shRNA lentivurs transfection prevents GP1a-induced increases in 5-HT2A receptor mRNA. **p<0.01, significant effect of GP1a treatment on 5-HT receptor mRNA levels in control shRNA tranfected cells compared 2A to vehicle-treated controls. ##p<0.01, significant effect of β-Arr2 shRNA transfection on the GP1a-induced upregulation of 5-HT2A receptors. Representative Western blots are shown in this figure and IOD was calculated as described in Experimental Procedures. The data represent mean ± SEM (n=6–8).

Journal: Pharmacological research : the official journal of the Italian Pharmacological Society

Article Title: Cannabinoid Agonists Increase the Interaction between ?-Arrestin 2 and ERK1/2 and Upregulate ?-Arrestin 2 and 5-HT 2A Receptors

doi: 10.1016/j.phrs.2012.11.002

Figure Lengend Snippet: (A) Enhanced immunoprecipitation of the ERK1/2 (Lane 4) compared to vehicle-treated controls (Lane 3). Negative controls (Lanes 5 and 6) received the same concentration of β-Arrestin 2 antibody except that the coupling resin was replaced with control agarose resin that is not amine reactive. All columns were incubated with prefrontal cortex lysate (300 μg) from vehicle (Lanes 3 and 5 ) or CP55940 (Lanes 4 and 6) treated rats. Prefrontal cortex lysate (30 μg of protein) was used as an input control (Lane 1 and 2). (B) Increased pERK protein levels in CP55940 treated rats compared to vehicle treated rats. **p<0.01, significant effect of CP55940 treatment compared to vehicle-treated controls. (C) Increased membrane associated β-Arrestin 2 protein levels in PFCx of CP55940 treated rats. **p<0.01 significant effect of CP55940 treatment compared to vehicle-treated controls. (D) CP55940 treatment does not affect total ERK1/2 expression in the PFCx. (E) Increased β-Arrestin 2 mRNA levels in PFCx of CP55940 treated rats. *p<0.01 significant effect of CP55940 treatment compared to vehicle treated controls. (F) β-Arr2 shRNA lentivurs transfection prevents GP1a-induced increases in 5-HT2A receptor mRNA. **p<0.01, significant effect of GP1a treatment on 5-HT receptor mRNA levels in control shRNA tranfected cells compared 2A to vehicle-treated controls. ##p<0.01, significant effect of β-Arr2 shRNA transfection on the GP1a-induced upregulation of 5-HT2A receptors. Representative Western blots are shown in this figure and IOD was calculated as described in Experimental Procedures. The data represent mean ± SEM (n=6–8).

Article Snippet: β-Arrestin 2 shRNA (r), CB 2 shRNA (r), CB 1 shRNA (r), copGFP control, control shRNA lentiviral particles, polybrene, and puromyocin were purchased from Santa Cruz, CA.

Techniques: Immunoprecipitation, Concentration Assay, Control, Incubation, Membrane, Expressing, shRNA, Transfection, Western Blot

(A) Reduced CB2 or CB1 mRNA levels in cells treated with CB2 or CB1 shRNA lentiviral particles, respectively. **p<0.01 significant effect of CB2 or CB1 shRNA compared to control shRNA treated cells. (B) CB2 shRNA lentivitrarus transfection prevents CP55940, JWH-133 and GP1a-induced increases in β-Arrestin 2 mRNA. **p<0.01, significant effect of CP55940, JWH-133, and GP1a treatment on β-Arrestin 2 mRNA levels in control shRNA lentivirus tranfected cells compared to vehicle-treated controls. ##p<0.01, significant effect of CB2 shRNA lentivirus transfection on the CP55940, JWH-133, and GP1a-induced upregulation of β-Arrestin 2. (C) CB1 shRNA lentivirus transfection does not prevent CP55940, JWH-133 or GP1a-induced increases β-Arrestin 2. **p<0.01, significant effect of CP55940, JWH-133 or GP1a treatment on β-Arrestin 2 mRNA levels in control or CB1 shRNA lentvirus treated cells compared to vehicle-treated controls. The data represent mean ± SEM (n=3).

Journal: Pharmacological research : the official journal of the Italian Pharmacological Society

Article Title: Cannabinoid Agonists Increase the Interaction between ?-Arrestin 2 and ERK1/2 and Upregulate ?-Arrestin 2 and 5-HT 2A Receptors

doi: 10.1016/j.phrs.2012.11.002

Figure Lengend Snippet: (A) Reduced CB2 or CB1 mRNA levels in cells treated with CB2 or CB1 shRNA lentiviral particles, respectively. **p<0.01 significant effect of CB2 or CB1 shRNA compared to control shRNA treated cells. (B) CB2 shRNA lentivitrarus transfection prevents CP55940, JWH-133 and GP1a-induced increases in β-Arrestin 2 mRNA. **p<0.01, significant effect of CP55940, JWH-133, and GP1a treatment on β-Arrestin 2 mRNA levels in control shRNA lentivirus tranfected cells compared to vehicle-treated controls. ##p<0.01, significant effect of CB2 shRNA lentivirus transfection on the CP55940, JWH-133, and GP1a-induced upregulation of β-Arrestin 2. (C) CB1 shRNA lentivirus transfection does not prevent CP55940, JWH-133 or GP1a-induced increases β-Arrestin 2. **p<0.01, significant effect of CP55940, JWH-133 or GP1a treatment on β-Arrestin 2 mRNA levels in control or CB1 shRNA lentvirus treated cells compared to vehicle-treated controls. The data represent mean ± SEM (n=3).

Article Snippet: β-Arrestin 2 shRNA (r), CB 2 shRNA (r), CB 1 shRNA (r), copGFP control, control shRNA lentiviral particles, polybrene, and puromyocin were purchased from Santa Cruz, CA.

Techniques: shRNA, Control, Transfection

(A) PD198306, potent ERK1/2 inhibitor, pretreatment prevents GP1a-induced increases β-Arrestin 2 mRNA levels. **p<0.01, significant effect of GP1a treatment compared to vehicle-treated controls. ##p<0.01, significant effect of PD198306 pretreatment on GP1a-induced increases β-Arrestin 2 mRNA levels compared to vehicle-treated controls (B) β-Arrestin 2 shRNA lentivirus treatment significantly reduced CP55940 induced increased in nuclear-associated pERK levels. **p<0.01, significant effect of CP55940 treatment on nuclear-associated pERK levels in control or β-Arrestin 2 shRNA treated cells compared to vehicle-treated. ##p<0.01, significant effect of β-Arrestin 2 shRNA treatment in CP55940 treated cells compared to control shRNA transfect/CP55940 treated cells. (C) β-Arrestin shRNA lentivirus treatment significantly reduced GP1a-induced increases in nuclear-associated pERK levels. **p<0.01, significant effect of GP1a treatment on nuclear-associated pERK levels in control or β-Arrestin 2 shRNA treated cells compared to vehicle-treated controls. ##p<0.01, significant effect of β-Arrestin 2 shRNA treatment in CP55940 treated cells compared to control shRNA transfected/CP55940 treated cells. The data represent mean ± SEM (n=3).

Journal: Pharmacological research : the official journal of the Italian Pharmacological Society

Article Title: Cannabinoid Agonists Increase the Interaction between ?-Arrestin 2 and ERK1/2 and Upregulate ?-Arrestin 2 and 5-HT 2A Receptors

doi: 10.1016/j.phrs.2012.11.002

Figure Lengend Snippet: (A) PD198306, potent ERK1/2 inhibitor, pretreatment prevents GP1a-induced increases β-Arrestin 2 mRNA levels. **p<0.01, significant effect of GP1a treatment compared to vehicle-treated controls. ##p<0.01, significant effect of PD198306 pretreatment on GP1a-induced increases β-Arrestin 2 mRNA levels compared to vehicle-treated controls (B) β-Arrestin 2 shRNA lentivirus treatment significantly reduced CP55940 induced increased in nuclear-associated pERK levels. **p<0.01, significant effect of CP55940 treatment on nuclear-associated pERK levels in control or β-Arrestin 2 shRNA treated cells compared to vehicle-treated. ##p<0.01, significant effect of β-Arrestin 2 shRNA treatment in CP55940 treated cells compared to control shRNA transfect/CP55940 treated cells. (C) β-Arrestin shRNA lentivirus treatment significantly reduced GP1a-induced increases in nuclear-associated pERK levels. **p<0.01, significant effect of GP1a treatment on nuclear-associated pERK levels in control or β-Arrestin 2 shRNA treated cells compared to vehicle-treated controls. ##p<0.01, significant effect of β-Arrestin 2 shRNA treatment in CP55940 treated cells compared to control shRNA transfected/CP55940 treated cells. The data represent mean ± SEM (n=3).

Article Snippet: β-Arrestin 2 shRNA (r), CB 2 shRNA (r), CB 1 shRNA (r), copGFP control, control shRNA lentiviral particles, polybrene, and puromyocin were purchased from Santa Cruz, CA.

Techniques: shRNA, Control, Transfection